The AMPure XP bead-based reagent is intended for performing DNA cleanup steps in different genomic applications like sequencing, qPCR/ddPCR/PCR, microarrays, and other enzymatic reactions. It is powered by proven SPRI technology, which uses paramagnetic beads to selectively bind nucleic acids by type and size.
- Works with DNA
- High recovery of amplicons, greater than 100 bp
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Predictable and consistent size selection
Maximizing recovery, consistency, and speed to facilitate the entire NGS workflow, the AMPure XP reagent meets the stringent needs of today’s genomic applications and minimizes the risk of losing important genetic information. That's why it's suggested in over 200 NGS library preparation kits often as the sole cleanup option, including those from the industry's most trusted sequencing companies like Illumina, Oxford Nanopore Technologies, PacBio and Thermo Scientific.
Specifications
- Nucleic Acid Input: PCR products, fragmented DNA
- Output: DNA
- Recovery: AMPure XP reagent was developed for the purification of PCR products 100bp<. Normal recovery ranges from 60-90%, and is typically between 70-90%
- Bead Ratio: 1.8X for cleanup
- Format: Liquid
- Volumes: 5 mL, 60 mL, or 450 mL
- Applications: PCR Purification, DNA Cleanup, NGS Cleanup
- Processing mode: Automated or manual
- Technology: SPRI paramagnetic bead-based technology
- Storage: 4 °C